Team:UC Davis E/Bioindustrial Enzyme
From 2012e.igem.org
Bioindustrial Enzyme
We then apply our proprietary technology to the pursuit of this target molecule. The key components of our industrial synthetic biology platform are strain engineering, process development, and scale-up.
Strain Engineering: The primary biological pathway within the microbe that we currently use to produce our target molecules is the isoprenoid pathway. Isoprenoids consist of a large, diverse class of molecules with current product applications in a wide range of industries, including specialty chemicals and fuels. Our platform utilizes proprietary high-throughput processes to create and test evolving strains in order to choose those specific strains that are most efficient and scalable.
Process Development and Scale-up: The basis of our production is a well-established fermentation process that uses our genetically-engineered microorganism strains to convert the plastic source into target molecules such as terephthalic acid. We will employ a multi-stage scale-up approach to progress from laboratory scale to commercial production scale. Once scaled up, AmberCycle scientists will maintain a constant feedback loop with or fermentation process and our laboratory, where strains are continuously created and modified, in hopes to expose those strains to conditions that simulate a commercial production environment. This allows us to focus our microbe development resources on those strains that demonstrate the highest potential to scale effectively.