"
Team:BIOSINT Mexico/operations
From 2012e.igem.org
| Line 142: | Line 142: | ||
<p class="clr-1"><strong>Operative cycle.</strong></p> | <p class="clr-1"><strong>Operative cycle.</strong></p> | ||
</div> | </div> | ||
| - | <p> | + | <p><div style="text-align: center;"><img src="http://www.persoft.mx/biosint/images/OPP.png"/></div></p> |
</div> | </div> | ||
| Line 156: | Line 156: | ||
With the Applied Biosystems ABI 394 DNA Synthesizer Kuxtal Biotech will be able to extend two or four oligonucleotides simultaneously at a rate of 12 bases per hour.</br></br> | With the Applied Biosystems ABI 394 DNA Synthesizer Kuxtal Biotech will be able to extend two or four oligonucleotides simultaneously at a rate of 12 bases per hour.</br></br> | ||
| - | <div style="text-align: center;"><img src="http://www.persoft.mx/biosint/images/Operational Plan Capacity.png" alt="Opereational plan" /></div> | + | <div style="text-align: center;"><img src="http://www.persoft.mx/biosint/images/Operational Plan Capacity.png" alt="Opereational plan" /></div> |
| + | Total capacity: 29 bases/hour x 24 hours/day = 696 bases /day = 35 primers of 20 bases produced per day.</br></br> | ||
</p> | </p> | ||
</div> | </div> | ||
| Line 162: | Line 163: | ||
With the Applied Biosystems ABI 394 DNA Synthesizer Kuxtal Biotech will be able to extend two or four oligonucleotides simultaneously at a rate of 12 bases per hour.</br></br> | With the Applied Biosystems ABI 394 DNA Synthesizer Kuxtal Biotech will be able to extend two or four oligonucleotides simultaneously at a rate of 12 bases per hour.</br></br> | ||
| - | <div style="text-align: center;"><img src="http://www.persoft.mx/biosint/images/OP2.png"></div | + | <div style="text-align: center;"><img src="http://www.persoft.mx/biosint/images/OP2.png"></div> |
Total capacity: 7.2 mers/hour x 24 hours/day = 172.8 bases /day = 8 primers of 20 mers produced per day.</br></br></p> | Total capacity: 7.2 mers/hour x 24 hours/day = 172.8 bases /day = 8 primers of 20 mers produced per day.</br></br></p> | ||
| Line 170: | Line 171: | ||
With the MerMade-192 E, DNA/RNA Synthesizer Kuxtal Biotech will be able to synthesize 192 primers in a single run of 3 hours for oligos of 20 mers.</br></br> | With the MerMade-192 E, DNA/RNA Synthesizer Kuxtal Biotech will be able to synthesize 192 primers in a single run of 3 hours for oligos of 20 mers.</br></br> | ||
| - | <div style="text-align: center;"><img src="http://www.persoft.mx/biosint/images/OP3.png"></div | + | <div style="text-align: center;"><img src="http://www.persoft.mx/biosint/images/OP3.png"></div> |
Total capacity: 768 mers/hour x 24 hours/day = 18432 bases /day = 921 primers of 20 mers produced per day.</br></br> | Total capacity: 768 mers/hour x 24 hours/day = 18432 bases /day = 921 primers of 20 mers produced per day.</br></br> | ||
With the MerMade- 192 E, DNA/RNA Synthesizer Kuxtal Biotech will be able to synthesize 192 primers in a single run of 3 hours for oligos of 20 mers.</br></br> | With the MerMade- 192 E, DNA/RNA Synthesizer Kuxtal Biotech will be able to synthesize 192 primers in a single run of 3 hours for oligos of 20 mers.</br></br> | ||
| - | <div style="text-align: center;"><img src="http://www.persoft.mx/biosint/images/OP4.png"></div | + | <div style="text-align: center;"><img src="http://www.persoft.mx/biosint/images/OP4.png"></div> |
Total capacity: 4 mers/hour x 24 hours/day = 96 bases /day = 4 primers of 20 mers produced per day. | Total capacity: 4 mers/hour x 24 hours/day = 96 bases /day = 4 primers of 20 mers produced per day. | ||
Revision as of 22:19, 27 October 2012
Location and infrastructure.
Kuxtal Biotech is going to be installed in Queretaro City, in the Industrial Park Benito Juárez, which is located in Av. 5 de febrero No. 1057, CP. 76130 (geographical coordinates: 20°37'40" N and 100°25'46" W and 1890 m above sea level).The climate in the region is tempered and semi-dry, with a maximum temperature of 35.2 ° C and minimum of 1.8 ° C, with an annual average of 17 °C. The construction is 200 m2 and the cost of the rent is 808 USD, it has offices with an area of 29m2, an access ramp and parking space for 4. The interior design includes: slab floor with epoxy paste, masonry walls covered with plaster and vinyl coatings.
Operative cycle.
Installed capacity.
To satisfy the products’ demand, Kuxtal Biotech will use two different synthesis equipments that will enable it to meet its production needs. Applied Byosistems ABI 394 Synthesizer will allow the synthesis of sequences that require specific conditions, while the MerMade-192 E, DNA/RNA Synthesizer will be use for oligos mass production. The daily production capacity analysis by equipment is presented below: With the Applied Biosystems ABI 394 DNA Synthesizer Kuxtal Biotech will be able to extend two or four oligonucleotides simultaneously at a rate of 12 bases per hour.
Quality assurance systems.
Kuxtal Biotech is looking for certifications like ISO 9001:2008 to ensure the quality of its management system and demonstrate its ability to consistently provide products or services that meet customer requirements; it also seeks continuous improvement and assurance legal and regulatory requirements. Among the technical requirements Kuxtal seeks to achieve the evaluation of personnel, facilities and environmental conditions, method validation, maintenance and calibration of equipment, quality of materials and reagents, quality assurance of results and its reporting.
