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I am reaching the end of my PhD on algal molecular biology and have found the CUO extremely useful. To date my use of it has fallen into three categories. My initial use of it was for the assisted design or redesign of several recombinant genes for expression in the Chlamydomonas reinhardtii chloroplast, a task which it has excelled at. Further to this I have used the program extensively for the analysis of previously expressed genes in the C. reinhardtii chloroplast to evaluate trends in expressing and non-expressing sub-sets. The CUO has also proven to be a very powerful tool for the study of the codon and codon pair usage in genes native to the C. reinhardtii chloroplast, a task I would have been unable to complete without this program.
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Testimonials
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I have used CUO to optimize several gene and tag sequences for expression in the Chlamydomonas chloroplast. The advantage of this program lies in its integration of codon usage with codon pairing optimization, using data from highly expressed genes. The interface is professional, flexible and easy to navigate and the manual provides clear instructions. Whilst we are still gathering data, early indications are that the program performs very well in redesigning genes for optimal expression.
